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Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol

Fertility and sterility, 2012-05, Vol.97 (5), p.1152-1157.e2 [Peer Reviewed Journal]

American Society for Reproductive Medicine ;2012 American Society for Reproductive Medicine ;2015 INIST-CNRS ;Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. ;ISSN: 0015-0282 ;EISSN: 1556-5653 ;DOI: 10.1016/j.fertnstert.2012.02.010 ;PMID: 22369773 ;CODEN: FESTAS

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Title:
Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol
Author: Baert, Yoni, M.Sc ; Goossens, Ellen, Ph.D ; van Saen, Dorien, M.Sc ; Ning, Liang, M.D ; in’t Veld, Peter, Ph.D ; Tournaye, Herman, M.D., Ph.D
Subjects: allografting ; Animals ; Biological and medical sciences ; Birth control ; Busulfan ; Cryopreservation ; Disease Models, Animal ; electron microscopy ; Fertility Preservation - adverse effects ; Fertility Preservation - methods ; freezing ; Graft Survival ; Green Fluorescent Proteins - biosynthesis ; Green Fluorescent Proteins - genetics ; Gynecology. Andrology. Obstetrics ; Immunohistochemistry ; Infertility, Male - chemically induced ; Infertility, Male - metabolism ; Infertility, Male - pathology ; Infertility, Male - physiopathology ; Infertility, Male - surgery ; Internal Medicine ; Male ; male infertility ; Medical sciences ; Mice ; Mice, Transgenic ; Microscopy, Electron, Transmission ; Obstetrics and Gynecology ; Organ Preservation - adverse effects ; Recovery of Function ; Sexual Development ; somatic cells ; Spermatogenesis ; Stem cells ; Sterility. Assisted procreation ; Testis - injuries ; Testis - metabolism ; Testis - physiopathology ; Testis - transplantation ; Testis - ultrastructure ; Time Factors ; transplantation ; ultrastructure ; Vitrification
Is Part Of: Fertility and sterility, 2012-05, Vol.97 (5), p.1152-1157.e2
Description: Objective To investigate whether solid-surface vitrification (SSV) is an effective cryopreservation strategy regarding the integrity and function of prepubertal mouse testicular tissue. Design Prospective experimental study. Setting Academic research unit. Animal(s) Mice. Intervention(s) Testicular tissue from 5- to 10-day-old GFP+ mice was cryopreserved with the use of a conventional uncontrolled slow freezing (USF) technique and SSV before intratesticular grafting in busulfan-treated GFP− mice. Main Outcome Measure(s) Ultrastructural cryoinjury to spermatogonial stem cells (SSCs) and somatic cells was assessed by electron microscopy. Tubular structure was evaluated by histology, and graft survival and spermatogenic recovery by immunohistochemistry. Result(s) The tubular morphology and the proportion of ultrastructural cryodamage were similar between vitrified and slow-frozen testicular fragments. Allografting of tissue after both USF and SSV resulted in a recovery of spermatogenesis similar to fresh samples. Conclusion(s) SSV resulted in success rates similar to USF in maintaining testicular cell ultrastructure, tubular morphology, and tissue function. These data provide further evidence that vitrification, being an inexpensive and simple technique, can be considered as an alternative for cryopreservation of prepubertal testicular tissue.
Publisher: New York, NY: Elsevier Inc
Language: English
Identifier: ISSN: 0015-0282
EISSN: 1556-5653
DOI: 10.1016/j.fertnstert.2012.02.010
PMID: 22369773
CODEN: FESTAS
Source: MEDLINE
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Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol

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