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Skeletal muscle-derived cells repair peripheral nerve defects in mice

Neural regeneration research, 2020-01, Vol.15 (1), p.152-161 [Peer Reviewed Journal]

COPYRIGHT 2020 Medknow Publications and Media Pvt. Ltd. ;2020. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;Copyright © Wanfang Data Co. Ltd. All Rights Reserved. ;Copyright: © Neural Regeneration Research 2020 ;ISSN: 1673-5374 ;EISSN: 1876-7958 ;DOI: 10.4103/1673-5374.264462 ;PMID: 31535664

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  • Title:
    Skeletal muscle-derived cells repair peripheral nerve defects in mice
  • Author: Chen, Zi-Xiang ; Lu, Hai-Bin ; Jin, Xiao-Lei ; Feng, Wei-Feng ; Yang, Xiao-Nan ; Qi, Zuo-Liang
  • Subjects: Care and treatment ; Catheters ; Defects ; Epoxy resins ; Health aspects ; House mouse ; Immunoglobulins ; Laboratory animals ; Microscopy ; Muscle cells ; muscle nerve conduit; myokine; nerve regeneration; nerve repair; peripheral nerve regeneration; polyurethane catheter; seed cells; skeletal muscle; skeletal muscle-derived cells; tissue-engineered nerve ; Musculoskeletal system ; Nervous system abnormalities ; Peripheral nervous system diseases ; Physiological aspects ; Skeletal muscle ; Skin ; Stem cell transplantation ; Stem cells ; Surgery
  • Is Part Of: Neural regeneration research, 2020-01, Vol.15 (1), p.152-161
  • Description: Skeletal muscle-derived cells have strong secretory function, while skeletal muscle-derived stem cells, which are included in muscle-derived cells, can differentiate into Schwann cell-like cells and other cell types. However, the effect of muscle-derived cells on peripheral nerve defects has not been reported. In this study, 5-mm-long nerve defects were created in the right sciatic nerves of mice to construct a peripheral nerve defect model. Adult female C57BL/6 mice were randomly divided into four groups. For the muscle-derived cell group, muscle-derived cells were injected into the catheter after the cut nerve ends were bridged with a polyurethane catheter. For external oblique muscle-fabricated nerve conduit and polyurethane groups, an external oblique muscle-fabricated nerve conduit or polyurethane catheter was used to bridge the cut nerve ends, respectively. For the sham group, the sciatic nerves on the right side were separated but not excised. At 8 and 12 weeks post-surgery, distributions of axons and myelin sheaths were observed, and the nerve diameter was calculated using immunofluorescence staining. The number, diameter, and thickness of myelinated nerve fibers were detected by toluidine blue staining and transmission electron microscopy. Muscle fiber area ratios were calculated by Masson's trichrome staining of gastrocnemius muscle sections. Sciatic functional index was recorded using walking footprint analysis at 4, 8, and 12 weeks after operation. The results showed that, at 8 and 12 weeks after surgery, myelin sheaths and axons of regenerating nerves were evenly distributed in the muscle-derived cell group. The number, diameter, and myelin sheath thickness of myelinated nerve fibers, as well as gastrocnemius muscle wet weight and muscle area ratio, were significantly higher in the muscle-derived cell group compared with the polyurethane group. At 4, 8, and 12 weeks post-surgery, sciatic functional index was notably increased in the muscle-derived cell group compared with the polyurethane group. These criteria of the muscle-derived cell group were not significantly different from the external oblique muscle-fabricated nerve conduit group. Collectively, these data suggest that muscle-derived cells effectively accelerated peripheral nerve regeneration. This study was approved by the Animal Ethics Committee of Plastic Surgery Hospital, Chinese Academy of Medical Sciences (approval No. 040) on September 28, 2016.
  • Publisher: India: Wolters Kluwer India Pvt. Ltd
  • Language: English;Chinese
  • Identifier: ISSN: 1673-5374
    EISSN: 1876-7958
    DOI: 10.4103/1673-5374.264462
    PMID: 31535664
  • Source: ProQuest One Psychology
    TestCollectionTL3OpenAccess
    GFMER Free Medical Journals
    PubMed Central
    ProQuest Central
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