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sup.1 H NMR studies distinguish the water soluble metabolomic profiles of untransformed and RAS-transformed cells

PeerJ (San Francisco, CA), 2016-06, Vol.4, p.e2104 [Peer Reviewed Journal]

COPYRIGHT 2016 COPYRIGHT 2012-2015 PeerJ, Inc / Public user content licensed CC-BY 4.0 unless otherwise specified. Content is available for free at https://peerj.com ;COPYRIGHT 2016 PeerJ. Ltd. ;ISSN: 2167-8359 ;EISSN: 2167-8359 ;DOI: 10.7717/peerj.2104

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  • Title:
    sup.1 H NMR studies distinguish the water soluble metabolomic profiles of untransformed and RAS-transformed cells
  • Author: Marks, Vered ; Munoz, Anisleidys ; Rai, Priyamvada ; Walls, Jamie D
  • Subjects: Metabolites
  • Is Part Of: PeerJ (San Francisco, CA), 2016-06, Vol.4, p.e2104
  • Description: Metabolomic profiling is an increasingly important method for identifying potential biomarkers in cancer cells with a view towards improved diagnosis and treatment. Nuclear magnetic resonance (NMR) provides a potentially noninvasive means to accurately characterize differences in the metabolomic profiles of cells. In this work, we use .sup.1 H NMR to measure the metabolomic profiles of water soluble metabolites extracted from isogenic control and oncogenic HRAS-, KRAS-, and NRAS-transduced BEAS2B lung epithelial cells to determine the robustness of NMR metabolomic profiling in detecting differences between the transformed cells and their untransformed counterparts as well as differences among the RAS-transformed cells. Unique metabolomic signatures between control and RAS-transformed cell lines as well as among the three RAS isoform-transformed lines were found by applying principal component analysis to the NMR data. This study provides a proof of principle demonstration that NMR-based metabolomic profiling can robustly distinguish untransformed and RAS-transformed cells as well as cells transformed with different RAS oncogenic isoforms. Thus, our data may potentially provide new diagnostic signatures for RAS-transformed cells.
  • Publisher: PeerJ. Ltd
  • Language: English
  • Identifier: ISSN: 2167-8359
    EISSN: 2167-8359
    DOI: 10.7717/peerj.2104
  • Source: GFMER Free Medical Journals
    PubMed Central
    Directory of Open Access Journals
    ROAD: Directory of Open Access Scholarly Resources
    ProQuest Central
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