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Role of Autophagy in Granulocyte-Colony Stimulating Factor Induced Anti-Apoptotic Effects in Diabetic Cardiomyopathy

Diabetes & metabolism journal, 2021-07, Vol.45 (4), p.594-605 [Peer Reviewed Journal]

COPYRIGHT(C) KYOBO BOOK CENTRE ALL RIGHTS RESERVED ;ISSN: 2233-6079 ;EISSN: 2233-6087

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  • Title:
    Role of Autophagy in Granulocyte-Colony Stimulating Factor Induced Anti-Apoptotic Effects in Diabetic Cardiomyopathy
  • Author: Guang-yin Shen ; Jeong-hun Shin ; Yi-sun Song ; Hyun-woo Joo ; In-hwa Park ; Jin-hee Seong ; Na-kyoung Shin ; A-hyeon Lee ; Young Jong Cho ; Yonggu Lee ; Young-hyo Lim ; Hyuck Kim ; Kyung-soo Kim
  • Subjects: Apoptosis ; Autophagy ; cardiac ; Diabetic cardiomyopathies ; Granulocyte colony-stimulating factor ; Myocytes
  • Is Part Of: Diabetes & metabolism journal, 2021-07, Vol.45 (4), p.594-605
  • Description: Background: We previously, reported that granulocyte-colony stimulating factor (G-CSF) reduces cardiomyocyte apoptosis in diabetic cardiomyopathy. However, the underlying mechanisms are not yet fully understood. Therefore, we investigated whether the mechanisms underlying of the anti-apoptotic effects of G-CSF were associated with autophagy using a rat model of diabetic cardiomyopathy. Methods: Diabetic cardiomyopathy was induced in rats through a high-fat diet combined with low-dose streptozotocin and the rats were then treated with G-CSF for 5 days. Rat H9c2 cardiac cells were cultured under high glucose conditions as an in vitro model of diabetic cardiomyopathy. The extent of apoptosis and protein levels related to autophagy (Beclin-1, microtubule-binding protein light chain 3 [LC3]-II/LC3-I ratio, and P62) were determined for both models. Autophagy determination was performed using an Autophagy Detection kit. Results: G-CSF significantly reduced cardiomyocyte apoptosis in the diabetic myocardium in vivo and led to an increase in Beclin-1 level and the LC3-II/LC3-I ratio, and decreased P62 level. Similarly, G-CSF suppressed apoptosis, increased Beclin-1 level and LC3-II/LC3-I ratio, and decreased P62 level in high glucose-induced H9c2 cardiac cells in vitro. These effects of G-CSF were abrogated by 3-methyladenine, an autophagy inhibitor. In addition, G-CSF significantly increased autophagic flux in vitro. Conclusion: Our results suggest that the anti-apoptotic effect of G-CSF might be significantly associated with the up-regulation of autophagy in diabetic cardiomyopathy.
  • Publisher: 대한당뇨병학회
  • Language: Korean
  • Identifier: ISSN: 2233-6079
    EISSN: 2233-6087
  • Source: KoreaMed Synapse
    PubMed Central
    KoreaMed Open Access
    DOAJ Directory of Open Access Journals
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