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Comparison of the Effects of Cuprizone on Demyelination in the Corpus Callosum and Hippocampal Progenitors in Young Adult and Aged Mice

Neurochemical research, 2022-04, Vol.47 (4), p.1073-1082 [Peer Reviewed Journal]

The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 ;2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. ;The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021. ;ISSN: 0364-3190 ;EISSN: 1573-6903 ;DOI: 10.1007/s11064-021-03506-8 ;PMID: 35060065

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Title:
Comparison of the Effects of Cuprizone on Demyelination in the Corpus Callosum and Hippocampal Progenitors in Young Adult and Aged Mice
Author: Hahn, Kyu Ri ; Kim, Woosuk ; Jung, Hyo Young ; Kwon, Hyun Jung ; Kim, Dae Won ; Hwang, In Koo ; Yoon, Yeo Sung
Subjects: Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Brain-derived neurotrophic factor ; Cell Biology ; Cells (biology) ; Corpus Callosum ; Cuprizone ; Cuprizone - toxicity ; Cyclic AMP response element-binding protein ; Demyelinating Diseases - chemically induced ; Demyelinating Diseases - metabolism ; Demyelination ; Dentate gyrus ; Diet ; Disease Models, Animal ; Doublecortin protein ; Fibers ; Hippocampus ; Hippocampus - metabolism ; Mice ; Mice, Inbred C57BL ; Microscopic analysis ; Myelin ; Myelin basic protein ; Neural stem cells ; Neuroblasts ; Neurochemistry ; Neurology ; Neurosciences ; Oligodendroglia ; Original Paper ; Progenitor cells ; Proteins ; Reduction ; Young adults
Is Part Of: Neurochemical research, 2022-04, Vol.47 (4), p.1073-1082
Description: Cuprizone is commonly used to induce neuronal demyelination in mice. In the present study, we compared the cuprizone-induced demyelination in the corpus callosum and investigated the effects of cuprizone on proliferating cells and neuroblasts in the dentate gyrus of young adult and aged mice. 5-week- and 23-month-old mice were fed a normal diet or a 0.2% cuprizone-enriched diet for 5 weeks. Mice fed a cuprizone-supplemented diet showed a significant reduction in myelin basic protein-positive structures in the corpus callosum, with the reduction in myelinated fibers being confirmed by electron microscopic analysis. In addition, we observed a marked increase in Ki67-positive proliferating cells and doublecortin-immunoreactive neuroblasts in young adult mice in response to cuprizone treatment, although not in aged mice, as the basal levels of these cells were significantly lower in these older mice. Furthermore, Ser133-phosphorylated cAMP response element-binding protein (pCREB)-positive nuclei and brain-derived neurotrophic factor (BDNF) protein levels were significantly reduced in young adult mice following cuprizone treatment in young adult, although again not in the aged mice. However, in both young adult and aged mice, there were no significant reductions in hippocampal mature neurons in response to cuprizone treatment. These observations indicate that in the mice of both age groups a cuprizone-supplemented diet contributes to an increase in demyelination in the corpus callosum and neural progenitor cells in the dentate gyrus, although the damage is more pronounced in young adult mice. This demyelination and reduction in neural progenitor cells may be associated with changes in the levels of BDNF and pCREB in the dentate gyrus.
Publisher: New York: Springer US
Language: English
Identifier: ISSN: 0364-3190
EISSN: 1573-6903
DOI: 10.1007/s11064-021-03506-8
PMID: 35060065
Source: MEDLINE
ProQuest Central

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Comparison of the Effects of Cuprizone on Demyelination in the Corpus Callosum and Hippocampal Progenitors in Young Adult and Aged Mice

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