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Porous extraction paddle: a solid-phase extraction technique for studying the urine metabolome

Rapid communications in mass spectrometry, 2016-12, Vol.30 (23), p.2462-2470 [Peer Reviewed Journal]

Copyright © 2016 John Wiley & Sons, Ltd. ;ISSN: 0951-4198 ;EISSN: 1097-0231 ;DOI: 10.1002/rcm.7739 ;PMID: 27624170

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  • Title:
    Porous extraction paddle: a solid-phase extraction technique for studying the urine metabolome
  • Author: Shao, Gang ; MacNeil, Michael ; Yao, Yuanyuan ; Giese, Roger W.
  • Subjects: Acetates ; Elution ; Exposure ; Extraction ; Methyl alcohol ; Motors ; Paddles ; Urine
  • Is Part Of: Rapid communications in mass spectrometry, 2016-12, Vol.30 (23), p.2462-2470
  • Description: Rationale A method was needed to accomplish solid‐phase extraction of a large urine volume in a convenient way where resources are limited, towards a goal of metabolome and xenobiotic exposome analysis at another, distant location. Methods A porous extraction paddle (PEP) was set up, comprising a porous nylon bag containing extraction particles that is flattened and immobilized between two stainless steel meshes. Stirring the PEP after attachment to a shaft of a motor mounted on the lid of the jar containing the urine accomplishes extraction. The bag contained a mixture of nonpolar and partly nonpolar particles to extract a diversity of corresponding compounds. Results Elution of a urine‐exposed, water‐washed PEP with aqueous methanol containing triethylammonium acetate (conditions intended to give a complete elution), followed by MALDI‐TOF/TOF‐MS, demonstrated that a diversity of compounds had been extracted ranging from uric acid to peptides. Conclusions The PEP allows the user to extract a large liquid sample in a jar simply by turning on a motor. The technique will be helpful in conducting metabolomics and xenobiotic exposome studies of urine, encouraging the extraction of large volumes to set up a convenient repository sample (e.g. 2 g of exposed adsorbent in a cryovial) for shipment and re‐analysis in various ways in the future, including scaled‐up isolation of unknown chemicals for identification. Copyright © 2016 John Wiley & Sons, Ltd.
  • Publisher: England: Blackwell Publishing Ltd
  • Language: English
  • Identifier: ISSN: 0951-4198
    EISSN: 1097-0231
    DOI: 10.1002/rcm.7739
    PMID: 27624170
  • Source: Alma/SFX Local Collection
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