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Alkaliphilic Endoxylanase from Lignocellulolytic Microbial Consortium Metagenome for Biobleaching of Eucalyptus Pulp

Journal of Microbiology and Biotechnology, 2012, 22(12), , pp.1636-1643 [Peer Reviewed Journal]

2014 INIST-CNRS ;ISSN: 1017-7825 ;EISSN: 1738-8872 ;DOI: 10.4014/jmb.1206.06044

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Title:
Alkaliphilic Endoxylanase from Lignocellulolytic Microbial Consortium Metagenome for Biobleaching of Eucalyptus Pulp
Author: Weerachavangkul, Chawannapak, Mahidol University, Salaya, Nakornpathom, Thailand ; Laothanachareon, Thanaporn, National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani, Thailand ; Boonyapakron, Katewadee, National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani, Thailand ; Wongwilaiwalin, Sarunyou, National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani, Thailand ; Nimchua, Thidarat, National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani, Thailand ; Eurwilaichitr, Lily, National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani, Thailand ; Pootanakit, Kusol, Mahidol University, Salaya, Nakornpathom, Thailand ; Igarashi, Yasuo, The University of Tokyo, Tokyo, Japan ; Champreda, Verawat, National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani, Thailand
Subjects: Biobleaching ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; metagenome ; microbial consortium ; PASTA ; PATE CELLULOSIQUE ; PULP ; pulp modification ; xylanase ; 생물학
Is Part Of: Journal of Microbiology and Biotechnology, 2012, 22(12), , pp.1636-1643
Description: Enzymatic pre-bleaching by modification of pulp fibers with xylanases is an attractive approach to reduce the consumption of toxic bleaching chemicals in the paper industry. In this study, an alkaliphilic endoxylanase gene was isolated from metagenomic DNA of a structurally stable thermophilic lignocellulose-degrading microbial consortium using amplification with conserved glycosyl hydrolase family 10 primers and subsequent genome walking. The full-length xylanase showed 78% sequence identity to an endo-β-1,4-xylanase of Clostridium phytofermentans and was expressed in a mature form with an N-terminal His∧6 tag fusion in Escherichia coli. The recombinant xylanase Xyn3F was thermotolerant and alkaliphilic, working optimally at 65-70℃ with an optimal pH at 9-10 and retaining greater than 80% activity at pH 9, 60℃ for 1 h. Xyn3F showed a V∧max of 2,327 IU/mg and K∧m of 3.5 mg/ml on birchwood xylan. Pre-bleaching of industrial eucalyptus pulp with no prior pH adjustment (pH 9) using Xyn3F at 50 IU/g dried pulp led to 4.5-5.1% increase in final pulp brightness and 90.4-102.4% increase in whiteness after a single-step hypochlorite bleaching over the untreated pulp, which allowed at least 20% decrease in hypochlorite consumption to achieve the same final bleaching indices. The alkaliphilic xylanase is promising for application in an environmentally friendly bleaching step of kraft and soda pulps with no requirement for pH adjustment, leading to improved economic feasibility of the process.
Publisher: Seoul: Korean Society for Applied Microbiology
Language: English
Identifier: ISSN: 1017-7825
EISSN: 1738-8872
DOI: 10.4014/jmb.1206.06044
Source: GFMER Free Medical Journals

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Alkaliphilic Endoxylanase from Lignocellulolytic Microbial Consortium Metagenome for Biobleaching of Eucalyptus Pulp

2014 INIST-CNRS ;ISSN: 1017-7825 ;EISSN: 1738-8872 ;DOI: 10.4014/jmb.1206.06044

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