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Genetic interaction mapping in mammalian cells using CRISPR interference

Nature methods, 2017-06, Vol.14 (6), p.577-580 [Peer Reviewed Journal]

COPYRIGHT 2017 Nature Publishing Group ;Copyright Nature Publishing Group Jun 2017 ;ISSN: 1548-7091 ;EISSN: 1548-7105 ;DOI: 10.1038/nmeth.4286 ;PMID: 28481362

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Title:
Genetic interaction mapping in mammalian cells using CRISPR interference
Author: Du, Dan ; Roguev, Assen ; Gordon, David E ; Chen, Meng ; Chen, Si-Han ; Shales, Michael ; Shen, John Paul ; Ideker, Trey ; Mali, Prashant ; Qi, Lei S ; Krogan, Nevan J
Subjects: Analysis ; Animals ; Cells ; Chromatin ; Chromosome mapping ; Chromosome Mapping - methods ; Clustered Regularly Interspaced Short Palindromic Repeats - genetics ; Combinatorial analysis ; CRISPR ; Epistasis, Genetic - genetics ; Gene mapping ; Genetics ; High-Throughput Nucleotide Sequencing ; Interference ; Mammalian cells ; Mammals ; Mapping ; Methods ; Mice ; Pools ; Protein Interaction Mapping - methods ; Protein-protein interactions
Is Part Of: Nature methods, 2017-06, Vol.14 (6), p.577-580
Description: We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein-protein-interaction data revealed a functional map of chromatin regulation.
Publisher: United States: Nature Publishing Group
Language: English
Identifier: ISSN: 1548-7091
EISSN: 1548-7105
DOI: 10.1038/nmeth.4286
PMID: 28481362
Source: AUTh Library subscriptions: ProQuest Central
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Genetic interaction mapping in mammalian cells using CRISPR interference

COPYRIGHT 2017 Nature Publishing Group ;Copyright Nature Publishing Group Jun 2017 ;ISSN: 1548-7091 ;EISSN: 1548-7105 ;DOI: 10.1038/nmeth.4286 ;PMID: 28481362

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