Language:

Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam

Malaria journal, 2022-12, Vol.21 (1), p.1-371 [Peer Reviewed Journal]

COPYRIGHT 2022 BioMed Central Ltd. ;2022. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;The Author(s) 2022 ;ISSN: 1475-2875 ;EISSN: 1475-2875 ;DOI: 10.1186/s12936-022-04399-w ;PMID: 36471315

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
Author: Thang, Ngo Duc ; Rovira-Vallbona, Eduard ; Binh, Nguyen Thi Huong ; Dung, Dang Viet ; Ngoc, Nguyen Thi Hong ; Long, Tran Khanh ; Duong, Tran Thanh ; Ma ; Edgel, Kimberly A
Subjects: Analysis ; Antigens ; Case management ; Diagnosis ; Diagnostics ; ELISA ; Enzyme-linked immunosorbent assay ; Evaluation ; Genes ; Genetic aspects ; Health facilities ; Histidine ; Histidine rich protein 2 ; Histidine rich protein 3 ; Human diseases ; Infections ; Light microscopy ; Malaria ; Medical screening ; Microscopy ; Nucleotide sequence ; Parasites ; Plasma levels ; Plasmodium falciparum ; Polymerase chain reaction ; Proteins ; Rapid Diagnostic Test ; rRNA 18S ; Sample size ; Surveying ; Symptoms ; User training ; Vector-borne diseases
Is Part Of: Malaria journal, 2022-12, Vol.21 (1), p.1-371
Description: Background Malaria rapid diagnostic tests (RDTs) remain the main point-of-care tests for diagnosis of symptomatic Plasmodium falciparum malaria in endemic areas. However, parasites with gene deletions in the most common RDT target, histidine rich protein 2 (pfhrp2/HRP2), can produce false-negative RDT results leading to inadequate case management. The objective of this study was to determine the prevalence of hrp2/3 deletions causing false-negative RDT results in Vietnam (Gia Lai and Dak Lak provinces). Methods Individuals presenting with malaria symptoms at health facilities were screened for P. falciparum infection using light microscopy and HRP2-RDT (SD Bioline Malaria Antigen Pf/Pv RDT, Abbott). Microscopically confirmed P. falciparum infections were analysed for parasite species by 18S rRNA qPCR, and pfhrp2 and pfhrp3 exon2 deletions were investigated by nested PCR. pfhrp2 amplicons were sequenced by the Sanger method and HRP2 plasma levels were determined by enzyme-linked immunosorbent assay (ELISA). Results The prevalence of false-negative RDT results among symptomatic cases was 5.6% (15/270). No pfhrp2 and pfhrp3 deletions were identified. False-negative RDT results were associated with lower parasite density (p = 0.005) and lower HRP2 plasma concentrations (p < 0.001), as compared to positive RDT. Conclusions The absence of hrp2/3 deletions detected in this survey suggests that HRP2-based malaria RDTs remain effective for the diagnosis of symptomatic P. falciparum malaria in Central Vietnam. Keywords: Malaria, Plasmodium falciparum, Diagnostics, Rapid Diagnostic Test, Histidine rich protein 2, Histidine rich protein 3, Gene deletion, Surveillance, Vietnam
Publisher: London: BioMed Central Ltd
Language: English
Identifier: ISSN: 1475-2875
EISSN: 1475-2875
DOI: 10.1186/s12936-022-04399-w
PMID: 36471315
Source: Open Access: DOAJ Directory of Open Access Journals
Open Access: PubMed Central
SpringerOpen
AUTh Library subscriptions: ProQuest Central
ROAD
GFMER Free Medical Journals
Coronavirus Research Database

Back to results list

Previous Result 14 Next Go to Next page


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam

Searching Remote Databases, Please Wait