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16S rRNA gene sequencing analysis of gut microbiome in a mini‐pig diabetes model

Animal models and experimental medicine, 2022-02, Vol.5 (1), p.81-88 [Peer Reviewed Journal]

2022 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences. ;2022 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences. ;2022. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 2576-2095 ;ISSN: 2096-5451 ;EISSN: 2576-2095 ;DOI: 10.1002/ame2.12202 ;PMID: 35213788

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Title:
16S rRNA gene sequencing analysis of gut microbiome in a mini‐pig diabetes model
Author: Niu, Miaomiao ; Zhao, Yuqiong ; Xiang, Lei ; Jia, Yunxiao ; Yuan, Jifang ; Dai, Xin ; Chen, Hua
Subjects: 16S rRNA ; Animals ; Bar codes ; Biosynthesis ; Cholesterol ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus - genetics ; Diet ; Digestive system ; Digestive tract ; Discriminant analysis ; Enterobactin ; Gastrointestinal Microbiome - genetics ; Genes, rRNA ; Glucose ; Glycolysis ; gut microbiome ; High density lipoprotein ; High fat diet ; Hogs ; Insulin ; Insulin resistance ; Intestinal microflora ; Intestine ; Lipids ; Lipoproteins ; Microbiomes ; Microbiota ; mini‐pig ; Nutrient deficiency ; Original ; Phylogenetics ; Regular ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sequence analysis ; Streptozocin ; Swine - microbiology ; Swine, Miniature - microbiology ; Taxonomy ; Tryptophan
Is Part Of: Animal models and experimental medicine, 2022-02, Vol.5 (1), p.81-88
Description: Background Currently, increasing attention is being paid to the important role of intestinal microbiome in diabetes. However, few studies have evaluated the characteristics of gut microbiome in diabetic miniature pigs, despite it being a good model animal for assessing diabetes. Methods In this study, a mini‐pig diabetes model (DM) was established by 9‐month high‐fat diet (HFD) combined with low‐dose streptozotocin, while the animals fed standard chow diet constituted the control group. 16S ribosomal RNA (rRNA) gene sequencing was performed to assess the characteristics of the intestinal microbiome in diabetic mini‐pigs. Results The results showed that microbial structure in diabetic mini‐pigs was altered, reflected by increases in levels of Coprococcus_3 and Clostridium_sensu_stricto_1, which were positively correlated with diabetes, and decreases in levels of the bacteria Rikenellaceae, Clostridiales_vadinBB60_group, and Bacteroidales_RF16_group, which were inversely correlated with blood glucose and insulin resistance. Moreover, PICRUSt‐predicted pathways related to the glycolysis and Entner‐Doudoroff superpathway, enterobactin biosynthesis, and the l‐tryptophan biosynthesis were significantly elevated in the DM group. Conclusion These results reveal the composition and predictive functions of the intestinal microbiome in the mini‐pig diabetes model, further verifying the relationship between HFD, gut microbiome, and diabetes, and providing novel insights into the application of the mini‐pig diabetes model in gut microbiome research. Microbial structure in diabetic mini‐pigs was altered, reflected by increasing in Lactobacillus, Peptococcus, Coprococcus_3, Clostridium_sensu_stricto_1, and Paludibacter, and decreasing in the bacteria Rikenellaceae_RC9_gut_group, Prevotella_7, and some unclassified genera of p_2534_18B5_gut_group, Clostridiales_vadinBB60_group, Bacteroidales_RF16_group, Izimaplasmatales, and Peptococcaceae.
Publisher: United States: John Wiley & Sons, Inc
Language: English
Identifier: ISSN: 2576-2095
ISSN: 2096-5451
EISSN: 2576-2095
DOI: 10.1002/ame2.12202
PMID: 35213788
Source: MEDLINE
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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16S rRNA gene sequencing analysis of gut microbiome in a mini‐pig diabetes model

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